Polyclonal antibodies (PAbs) are powerful biological tools that are used in the analysis of antigens in a wide variety of research and in vitro diagnostic applications. Traditionally, polyclonal antibodies are raised in large animals such as goats and rabbits. The immunized animal produces an antibody response that is heterogeneous in nature and the serum harvested recognises multiple antigenic epitopes. Polyclonal antibodies can be generated more rapidly than monoclonal antibodies (MAbs) and consequently a vast number of polyclonal antibodies have been developed against a wide range of biological targets. In recent years, the use of affinity purification has improved the quality and performance of polyclonal antibodies. Like Monoclonal antibodies, PAbs are used in a wide variety of applications including western blotting, Enzyme linked immunosorbent assays (ELISA), immunohistology and immunoprecipitation techniques. Polyclonal antibodies are more stable than MAbs over a broad pH and are therefore more suitable for pH-sensitive applications.
ARP American Research Products provides an extensive range of internationally recognised polyclonal antibodies. The product range includes high-quality antibodies, designed for research use, against biological targets that are of potential diagnostic and therapeutic interest.
Polyclonal antibodies recognising complement components
The complement system consists of a specific group of proteins that play a pivotal role in the innate immune response, inflammation and homeostasis. In response to the recognition of a potential pathogen, inactive complement proteins interact with each other in an enzymatic cascade and become sequentially activated. Protein fragments are generated during this process that acts as chemo-attractants, adhesion molecules and opsonins. The complement system can be activated via three different pathways 1) Classical pathway 2) alternative pathway 3) Mannose-binding lectin pathway.
ARP American Research Products supplies a wide range of polyclonal antibodies that recognise key components of the complement system including C1q and Cd4.
- 12-5000 Rabbit anti human C4d
- 12-5050 Rabbit anti human C4d: Ready-to-use
- 21-1131 Goat anti C1q conjugated to FITC
Polyclonal anti p62 protein
The p62 protein, also known as sequestosome 1, is encoded by the SQSTM1 gene. It is an adaptor protein that binds ubiquitin and is involved in bone remodeling and various cellular processes. P62 also acts as an autophagy receptor that is required for the formation and degradation of ubiquitin.
ARP supplies a range of polyclonal antibodies recognising the p62 protein including a guinea pig polyclonal against the C-terminal region of p62.
Polyclonal antibodies recognising Adeno-associated virus (AAV)
Adeno-associated viruses A(AAV), from the parvovirus family, are small single-stranded DNA viruses that affect humans and some additional primate species. AAV is currently considered to be non-pathogenic and most people infected with AAV only develop a mild immune response. Adeno-associated viruses carry genes into both dividing and quiescent cells where they can insert genetic information into the host genome These characteristics of AAV have been exploited for gene therapy and AAV virus have been engineered to carry therapeutic genes into host cells. A range of polyclonal antibodies is available from ARP which recognise intact AAV particles and capsid proteins VP1, VP2 and VP3.
- 03-61084 Rabbit anti Adeno-associated virus (AAV) VP1, VP2, VP3